The Primary Structure of Staphylococcal Enterotoxin B I. ISOLATION, COMPOSITION, AND SEQUEKCE OF TRYPTIC PEPTIDES FROM OXIDIZED EKTERO-

نویسندگان

  • I - YIH HUANG
  • MERLIN S. BERGDOLL
چکیده

Thirty peptides obtained from the tryptic digestion of oxidized staphylococcal enterotoxin B were isolated chromatographically on columns of Dowex SOW-X2 and AGl-X2 by using pyridine-acetic acid and N-ethylmorpholine-pyridine-acetic acid buffer systems. The isolated peptides were further purified by paper chromatography and electrophoresis. The amino acid composition and sequence of each purified peptide were determined. A total of 239 amino acid residues was found to be present in the tryptic peptides.

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تاریخ انتشار 2003